lipofectamine 3000 (Thermo Fisher)
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lipofectamine 3000
Lipofectamine 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine/Lapatinib/pmc13273694-118-18-20
Average 94 stars, based on 1 article reviews
Lipofectamine 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine/Lapatinib/pmc13273694-118-18-20
Average 94 stars, based on 1 article reviews
lipofectamine 3000 - by Bioz Stars,
2026-10
94/100 stars
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Transfection:Article Title: PSME3 regulates migration and differentiation of myoblasts. Article Snippet: For transfection with siRNA, cycling C2C12 cells were reversetransfected on two consecutive days with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) and either SMARTpool siRNA from Horizon Discovery targeting Psme3 (L062727-01-0005), NudC (L-041603-01-0005), or a nontargeting control pool (DH-D-001810-10-20) in Opti-MEM at 50 nM as described below. .. On the first day of transfection, 15 μl of 20 μM siRNA solution and 15 μl of Article Title: Synergistic targeting of cancer cells through simultaneous inhibition of key metabolic enzymes. Article Snippet: Target gene expression was silenced using small interfering RNAs (siRNAs) obtained from OriGene. .. Eukaryotic cells were transfected with Article Title: Hepatic miR-149-5p upregulation fosters steatosis, inflammation and fibrosis development in mice and in human liver organoids Article Snippet: Huh7 cells were transfected 24 hours after seeding using Interferin (Polyplus transfection, Illkirch, France) and Optimem (Gibco), following manufacturer’s instructions. .. HLOs were transfected using Incubation:Article Title: PSME3 regulates migration and differentiation of myoblasts. Article Snippet: For transfection with siRNA, cycling C2C12 cells were reversetransfected on two consecutive days with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) and either SMARTpool siRNA from Horizon Discovery targeting Psme3 (L062727-01-0005), NudC (L-041603-01-0005), or a nontargeting control pool (DH-D-001810-10-20) in Opti-MEM at 50 nM as described below. .. On the first day of transfection, 15 μl of 20 μM siRNA solution and 15 μl of Retroviral:Article Title: Article Snippet: .. Supplementary Information - page 8 of 18 Reconstituted SHP2-deficient Jurkat cells with SHP2 mutants J.SHP2KO cells underwent retroviral transduction with viruses expressing SHP2WT, SHP2T42A, or SHP2E76K, by spinning down at room temperature for 1 hour at 2,200 rpm (spinfection), in media supplemented with Transduction:Article Title: Article Snippet: .. Supplementary Information - page 8 of 18 Reconstituted SHP2-deficient Jurkat cells with SHP2 mutants J.SHP2KO cells underwent retroviral transduction with viruses expressing SHP2WT, SHP2T42A, or SHP2E76K, by spinning down at room temperature for 1 hour at 2,200 rpm (spinfection), in media supplemented with Expressing:Article Title: Article Snippet: .. Supplementary Information - page 8 of 18 Reconstituted SHP2-deficient Jurkat cells with SHP2 mutants J.SHP2KO cells underwent retroviral transduction with viruses expressing SHP2WT, SHP2T42A, or SHP2E76K, by spinning down at room temperature for 1 hour at 2,200 rpm (spinfection), in media supplemented with Saline:Article Title: Bright and pH-sensitive Baby Spinach aptamer with RNA triplex fusion Article Snippet: .. After a 24 h cultivation at 37°C with 5% CO 2 , cells were washed with phosphate-buffered saline (PBS; NaCl 130 mM, KCl 2.7 mM, Na 2 HPO 4 8 mM, KH 2 PO 4 2 mM) and transformed with 600 ng of plasmids using Transformation Assay:Article Title: Bright and pH-sensitive Baby Spinach aptamer with RNA triplex fusion Article Snippet: .. After a 24 h cultivation at 37°C with 5% CO 2 , cells were washed with phosphate-buffered saline (PBS; NaCl 130 mM, KCl 2.7 mM, Na 2 HPO 4 8 mM, KH 2 PO 4 2 mM) and transformed with 600 ng of plasmids using other:Article Title: Article Snippet: LPS stimulations were done with 100 ng/ml LPS (InvivoGen) for RAW 264.7 macrophages and 10ng/ml LPS for BMDMs for times indicated. Article Title: Neuropilin-2 functions as a coinhibitory receptor to regulate antigen-induced inflammation and allograft rejection Article Snippet: Briefly, U87MG cells (ATCC) were cultured in EMEM supplemented with 10% FBS (MilliporeSigma) and 100 U/mL penicillin/streptomycin (Lonza) as described ( ). |